报告题目：Rewriting Natural Decoding Rules by de novo Genome Synthesis
报告人：王开航 博士
主持人：何向伟 教授
时 间：2017年3月22日（周三）下午4点
地 点：纳米楼457报告厅
报告人简介：
王开航博士本科毕业于英国伦敦大学学院，博士毕业于英国剑桥大学，现为英国医学研究理事会分子生物学实验室高级研究人员（Senior Investigator Scientist）。
王开航博士主要致力于合成生物学研究，通过纯人工合成基因组来以人的意志设计并制造生命，以此来从本质突破一些地球生命进化的极限。其研究可以应用到很多不同领域，包括生物制药工程，干细胞研究，表观生物学，化学生物学等等。
王博士现以第一作者兼并列通讯作者身份在国际知名期刊Nature、Nature Chem、Angew Chem Int Edit 发表数篇研究论文。
讲座摘要:
Unnatural amino acids could be incorporated to expand the chemical, physical and biological properties of proteins to expand the function of life itself. There are two conceptual possibilities to achieve this goal: i). creation of orthogonal decoding rules running independently to the wildtype decoding rules to incorporate unnatural amino acids; and ii). de novo synthesis of a new genome withartificially redefined synthetic decoding rules to expand natural decoding capacities to include unnatural amino acids.
To explore the first possibility, the orthogonal ribosome andmultiple tRNAs were co-evolved to efficiently decodecognate quadruplet codons on an orthogonal mRNAs for incorporation of multiple distinct unnatural amino acids.This established the possibility to enhance the efficiency of unnatural amino acid incorporation in response to a synthetic orthogonal quadruplet codes, creating maximally 44=256 new quadruplet codons for unnatural amino acid incorporation.
The second possibility requires the de novo construction of a synthetic genome with re-programmed synthetic decoding rules to re-build a synthetic life that fundamentally go beyond the limits of the 20 natural amino acid side chains.To pave the foundation to reach for this goal, an efficient, specific, and iterative method in E. coli to replace defined genomic fragment with synthetic sequence (replicon excision enhanced recombination, REXER) has been developed; together with a feasible, modular, and scalable route for de novo genome synthesis (genomestepwise interchange synthesis, GENESIS), which allows the replacement of the entire 4.6-mb E. coli genome with synthetic DNA in around 15 or less iterated REXER steps.
